Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKISTYENKKVLVLGLAKSGV-SAAKLLHELGALVTVNDGKPFDENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITEVELAYEVA-ECPIIGITGTNGKTTTTTMTGLLLNAGADQGI-ARLAGNIGYPASGVAQEAKSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHG-TRKEYVKAKWNLQKNMTEKDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVY---A--DD--YSIYYKKEKIMEISELGVPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGE--------IQG-RKFYNDSKATNILATKMALSGFET----SKVVLLAGGL----DRGNTFDELIPSLKGIKAMVVFG-----------QTKEKLMDAGKKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVNRLK 2F00 Chain:B ((13-469)) --VPEMRRVRHIHFVGIGGAGMGGIAEVLANEGYQISGSDLAPNPVTQ-QLM--NLGATIYFN-HRPENV-RDASVVVVSSAISADNPEIVAAHEARIPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYA-EA-GLDPTFVNGGLV-KAAGVHARLGHGRYLIAEADESDASFL-HLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFLHNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLRQDK-EPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEFPLEPVNGKSGTAMLVDDY-GHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDL-YDDFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEMLAPVLTGNDLILVQGAGNIG---------------------

General information: TITO was launched using: RESULT: Template: 2F00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2406 -6318 -2.63 -15.11 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -2.63 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_2F00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F00-query.scw PDB file : Tito_Scwrl_2F00.pdb: