Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINLGIIGTNWITHQFVQAALSTK--KYELTAVYSRHLEKAQEFGSHYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPKEMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLP--LKDEIIGANFSYMKYSSRYDQVLDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGM--PKEVHYFPRKISTGVDGLGWGILRYENF-DVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNA-IEQAQFHDRAHKEII--DL--AVTAAENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNEEDF 2POQ Chain:X ((2-332)) ALRWGIVSVGLISSDFTAVLQTLPRSEHQVVAVAARDLSRAKEFAQKHDI-PKAYGSYEELAKDPNVEVAYVGTQHPQHKAAVMLCLAAGKAVLCEKPMGVNAAEVREMVTEARSRGLFLMEAIWTRFFPASEALRSVLAQGTLGDLRVARAEFGKNLTH--------VPRAVDWAQAGGALLDLGIYCVQFISMVFGGQKPEKISVMGRRHETGVDDTVTVLLQYPGEVHGSFTCSITAQLSNTASVSGTKGMAQLLNPCWCPTELVVKGEHKEFLLPPVPKNCNFDNGAGMSYEAKHVRECLRKGL----KESPVIPLVESELLADILEEVRRAIGVTFPQD-----

General information: TITO was launched using: RESULT: Template: 2POQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1889 -18747 -9.92 -58.77 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain X : 0.71 3D Compatibility (PKB) : -9.92 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_2POQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2POQ-query.scw PDB file : Tito_Scwrl_2POQ.pdb: