Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIFSTNTCPVPNDLESVIHHKDEVPAELCGMSEQQVQKIWKSIESLYKTGNYPLITFCLRRQGKILLNRSIGYAQGNSPAGLQENALIATPDTPVCLFSASKMITAMLIHLLDEKGEINLLDPVSYYIPEYGVNGKRRATIFHLLSHRGGIPYVDGDVTPELLFDKDEILRRLYAARPVSPAGNHLAYHAVTAGYILGEIIERVTGQDLRKFVHETIEKPMDMPYFNYGLKPEYRAEVALNCATGLHPRLGTDHYLNHVLGGGLQLAVDVTNDSRFMDTICPAGNIYTSAEQAGRFFEMLLSGGSYQGKQILSEKTIFRATLPTTGVNIDRTLLIPMRYALGPMLGSNPVGL------------------------FGPMTGQAFGHLGFS---NILCWADPERDISVSLLTTGKSVVGTHLPALANLLYQISIQCPRIPRDQRRSLFGSDSHETDLV 5EVL Chain:A ((8-367)) -------------------------------GKSDLDAAVEATIPPLMKAKDIPGMAVAVLADGKAHY-FNYGVASRET-------GQPVTQDTLFELGSISKTFTGILGGYALAQGKLSLADKASRYQPELKGSVFDRVSLLQLATYSAGGLPLQFP---DAVAGQASMLAYYRGWKPDYAPGERRLYSNPSI-GLFGHLAARSLGQPFDQAMERGLLPKLGLSHTFIHVPEAEQSRYAWGYA---------------------------------GVLDAEAYGIKSSAADLLKYLAINMSPPA-------DPALQRALDASHAAYY----RVGDMRQGLGWEGYRYPISLERLLAGNSNEIAFQPQKVEWLNPPRLAEGDVLLNKTGSTSGFGAYVLFVPARKVGIVMLANRNYPNAERV----RAAYRILSAVDPSL------------------

General information: TITO was launched using: RESULT: Template: 5EVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1806 -145572 -80.60 -450.69 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -80.60 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_5EVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EVL-query.scw PDB file : Tito_Scwrl_5EVL.pdb: