Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHYTGIDQLINSFDQALRSLVPGATAAQRQNPAETVEAKLGVEDARHVAGLMRVNHSGEVCAQALYHGQALTAKL---PNVRREMQQAAIEEQDHLAWCEDRLKELNSHTSLLNP-IWYGLSYGMGALAGIAGDKYSLGFVAETERQVSLHLQDHLNQLPAQDERSRKILEQMNEDELHHRHTALEAGGVELPYAVKITMTAISKLMTKTSYYL 2YW6 Chain:B ((11-155)) ------------------------------------------DKKASDVADLLQKQLSTYNDLHLTLKHVHWNVVGPNFIGVHEMIDPQVELVRGYADEVAERIATLGKSPKGTPGAIIKDRTWDDYS-VERDTVQAHLAALDLVYNGVIEDTRKSIEKLEDLDLVSQDLLIAHAGELEKFQWFVRAH---------------------------

General information: TITO was launched using: RESULT: Template: 2YW6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 551 -4052 -7.35 -28.73 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : -7.35 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_2YW6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YW6-query.scw PDB file : Tito_Scwrl_2YW6.pdb: