Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSTAKHNYAPDILGAGYEQLTLNFPDDYEGKVVATLVRKKATQPTQKAVLYIHGFLDYFFQ-TEMAEQFNAHGYDFYALDLRKYGRSKLPHQIF-YNVLDLIEYDAEITQALEIIGQEKHTQVLLAGHSTGGLTATLYAAHHPDHPLIKALWANSPFYDFNLSLVEKKFGIPMLSRVGKYLPKVKFPSQLNKWYTTSLHKQLKGEWDFNLDWKPTSAPTVQLSFVHAIHTAQKEIHRGVKLNIPALIMHSHQTKNPRKWGPDA------TQSDVILDVKDIAKFGKKMKGDVSVVSIHNGLHDLVLSAQPVREQVYQQLFQWLDQKVT 1Y37 Chain:A ((1-294)) --------------MFEGFERRLVDV-----GDVTINCVVGG----SGPALLLLHGFPQNLHMWARVAPLLAN-EYTVVCADLRGYGGSSKPVGAPDHANYSFRAMASDQRELMRTLGFER---FHLVGHDRGGRTGHRMALDHPDS-VLSLAVLDIIP----TYVMFEEVDRFVARAYWHWYFLQQPAPYPEKVIGADPDTFYEGCLFGWGATGADGFDPEQLEEYRKQWRDPAAIHGSCCDYRAGGTIDFELDHGDLGRQVQCPALVFSGSAGLMHSLFEMQVVWAPRLANMRFASLPGG-HFFVDRF---PDDTARILREFLSDARS

General information: TITO was launched using: RESULT: Template: 1Y37.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1693 -38436 -22.70 -134.39 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -22.70 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_1Y37.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y37-query.scw PDB file : Tito_Scwrl_1Y37.pdb: