Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNATLNYQPHWVREDFVDFVLQKINATWAWKRVLAEVTAVQSLHTDMVLIKLKPNRNFNFDQVRAGESILLTLLIDG-----------------------------VYQQRSYSIIEVT-TQGEIALGIKVQ-----------------GLVSRAAQLLHVGECVEISQPQGDFTLHQGQQPAILIASGSGVTAIYSLLQQALKQQ-L-EQIHVIYFNRA---EIFHAELKALAEQYPQLQYHFFNTTEQK--------QHLTESLLQKLVP---DFEQTATYVCGHHGMMQQANEIYTQKGAQ-SQLHQEYFQPLQVTGTHAAQPVIFRRAQQEFLAETNLLGSAEQAGLRPQHGCRMGVCNKCSCTKVSGVTQNLLTGEIEDQPNRPIKLCVSQALSPVTIDL 2R6H Chain:C ((8-290)) ------------------------------KEWECEVLSNKNVSTFIKEFVVKLPEG-ETMNFKSGSYAQIKIPKYNIRYADYDIQDRFRGDWDKMDAWSLTCKNEEETVRAYSMANYPAEGNIITLNVRIATPPFDRAANKWKAGIKPGISSSYIFSLKPGDKVMMSGPYGDFHIQDTDAEMLYIGGGAGMAPLRAQILHLFRTLKTGRKVSYWYGARSKNEIFYEEDFREIEREFPNFKFHIALSDPQPEDNWTGYVGFIHQVIYDNYLKDHDAPEDIEYYMCGPGPMANAVKGMLENLGVPRNMLFFDDFG-------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2R6H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1100 -6064 -5.51 -27.69 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : -5.51 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_2R6H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R6H-query.scw PDB file : Tito_Scwrl_2R6H.pdb: