Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDQSTTSKARPMTSTQGSWFAILAVAIVAFALVTSEFLPVG---VLNSVAADL-H----------ISVGTAGLIITVPGIMAAIAAPLLPVSVKQLDRRYVLILLTAIMVIANTITA---FAENFHVLLLSRLILGISIGGFWATAIALSGKLAPANLPIAKATAVVMAGVTFATVLGVPIGTWLS--EFYGWRSAFGITAAIGLVVLVLQLIFLPKLLPESA----IHI----------------------------------RDLPALLRTPKARSGMLIVLLIGLAHFCAYSYLA-PFFKNV-AGFNGTTISSLLLLYGIAGIFGNAFAGYSGNLNVRYTLAFVG-TCFAIVFFGFPIF----AIHEFGAIVLTALWGFAFGAFPTSANIWMFVHAPH-AVEKGMPLFVGMFQVMIATGSLLGGYIVDHFNENTLIYGVLSFVALALISTFTLAKGLNNPKATCEN 5C65 Chain:A ((6-432)) -------------VTPALIFAITVATIGSFQFGYNTGVINAPEKIIKEFIQKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTAL-RGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAA-LPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDA----PIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLGAYVF-------------------------------

General information: TITO was launched using: RESULT: Template: 5C65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1448 -176746 -122.06 -499.28 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -122.06 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_5C65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C65-query.scw PDB file : Tito_Scwrl_5C65.pdb: