Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRPIYLDYAATTPVDPQVAERMMECLTFDGTFGNAASRSHAYGWQAEEKVEYAREQVANLIKADPREIVWTSGATESDNLALKGVAQFYASKGKHIITSKIEHKAVLDPCRELEEQGFEITYLEPEPQTGLITPEMVKAALRPDTILVSLMMVNNEIGTVTDVAVIGELTRA--NKTFFHVDAAQAAGKVDIDLSTMKIDLMSFSAHKIYGPKGIGALYVRRSPRVRLKAQIHGGGHERGMRSGTLATHQIVGMGEAFELAGKTMHAEQERIRKLRDKLWNGLQDLEQVFLNGHPTQNVANYLNVSFNFVEGESLMMSLK--DAAVSSGSACTSATLEPSYVLRALGLSDELAHSSIRFSFGKYTTEEDIDHVLTITKAAVEKLRELSPLWDMYKEGIDLSTVEWAEH 1EG5 Chain:A ((1-376)) --MRVYFDNNATTRVDDRVLEEMIVFYRE--KYGNPNS-AHGMGIEANLHMEKAREKVAKVLGVSPSEIFFTSCATESINWILKTVAETFEKRKRTIITTPIEHKAVLETMKYLSMKGFKVKYVPVD-SRGVVKLEELEKLVDEDTFLVSIMAANNEVGTIQPVEDVTRIVKKKNKETLVHVDAVQTIGKIPFSLEKLEVDYASFSAHKFHGPKGVGITYIRKGV--PIRPLIHGGGQERGLRSGTQNVPGIVGAARAMEIAVEELSEAAKHMEKLRSKLVSGLMNLG-AHIITPLEISLPNTLSVSFPNIRGSTLQNLLSGYGIYVST------------HVLDAMGVDRRIAQGAIRISLCKYNTEEEVDYFLKKIEEILSFL------------------------

General information: TITO was launched using: RESULT: Template: 1EG5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2101 -67224 -32.00 -186.73 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -32.00 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_1EG5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EG5-query.scw PDB file : Tito_Scwrl_1EG5.pdb: