Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKFLNPQYLFQLLSPCLLCEIGTREKYSLCKECWEQLPWLKQTIQRNDQSVLVACHYAYPINRIIQQFKYEQKLHYQTLLAEVLQQLKF---PKVQAIVPMPISKQRLTERGFNQSLLLANLLGKQLKIPVWQPVQRLNEHSQK----GLSRLERFENIEQQFVALTQENRRYRRVLIIDDVITTGSSIHALSQALKQLGCTSIHASCLAAASSTSY 5VN4 Chain:B ((50-186)) ----------------------------------------------------------------FAEVGNITENPEVMRGIRDFFVDRYKNLQQPITHILGFDSR-------GF----LLGPMIAVELNVPFVL-IRKANKIAGVIIKSEPYTKEY----EECMTVRFGSFDKNSRVVLIDDVIATGGTMLAGVQLVDACGATLVEVAGILGL-----

General information: TITO was launched using: RESULT: Template: 5VN4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 567 -31544 -55.63 -250.35 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -55.63 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_5VN4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VN4-query.scw PDB file : Tito_Scwrl_5VN4.pdb: