Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHSDTLSLSLELLQQPSVTP------IDHTCQTIMADRLAKVGFHIEPMRF-----------GDVDNLWARRG-T-EGPVFCFAGHTDVVPTGRLDAWNSDPFAPEIRDGKLYGRGSADMKTALAAMVVASERFVAKHPNHKGSIAFLITSDEEGPAVNGTVKVIETLEKR-NEKITWCLVGEPSSTHKLGDIVKNGRRGSLNAVLK--VQGKQGHVAYPHL-ARNPIHEASPALAELCQTVWD---NG----------------------------------------N----EYFPATSFQISNIHA----GTGATNVIPGTLEVTFNFRYSTEVTAEQLKQRVHEILDKHG------LQYEIVWNLSGLPFLTPV-GELVNAAQTAILNVTGTETELSTSGGTSDGRF-IA-PTGAQVLELGVLN--ATIHQINEHVDVHDLDPLTDIYEQILENLLA 2ZOG Chain:A ((18-475)) -NQDRYVKKLAEWVAIQSVSAWPEKRGEIRRMMEVAAADVQRLGGSVELVDIGKQKLPDGSEIPLPPILLGKLGSDPQKKTVCIYGHLDVQPAALEDGWDSEPFTLVEREGKLYGRGSTDDKGPVAGWMNALEAYQKTGQEIPVNLRFCLEGMEESG-SEGLDELIFAQKDKFFKDVDYVCISDNYWLGKNKPCITYGLRGICYFFIEVECSDKDLHSGVYGGSVHEAMTDLISLMGCLVDKKGKILIPGINDAVAPVTDEEHALYDHIDFDMEEFAKDVGAETLLHSCKKDILMHRWRYPSLSLHGIEGAFSGS-GAKTVIPRKVVGKFSIRLVPDMIPEVVSEQVSSYLSKKFAELQSPNKFKVYMGHGGKPWVSDFNHPHYQAGRRALKTVFGVEPDLTREGGSIPVTLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRLNYIEGTKMLAAYLYEVS-

General information: TITO was launched using: RESULT: Template: 2ZOG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1670 44045 26.37 118.08 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 26.37 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_2ZOG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZOG-query.scw PDB file : Tito_Scwrl_2ZOG.pdb: