Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPFVLYNSEQRKKVEFVPRKEGHIDMYVC--GMTVYD-YCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDIDDKIIKRANENGETIQQLTMRFIDAMNEDAANLGCLAPDEA--PKAT--EYIDQMQNMIGNLVNKGAAYPASNGDVYFEVTKFEKYGRLSGRKLDDMQAGA-----SE-RVDV----E-VEKKH-PFD------------------FVLWKHA---------KEN--EPSWASPWGNGRPGWHIE-CSA-----------------------------------------------------MSTCCL-------------------------GNHFDIHGGGSDLMFPHHENEIA---QSEAS--T-GEQYVNYWIHVGFINVD-GEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSSH-YRSPVNFSDVALKEAKTSLTRFYHSFKAYQQVY---G----QTTTETLDQSFVERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQADQATVLYSTLRHLTNILGLVQHNVDDFLKSDIGQEALALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD 3ZIU Chain:B ((28-520)) ----------------FKTTNKSKDKFYALDMFPYPSGSGLHVGHPEGYTATDIISRYKRLKGFDVLHPIGWDAFGLPAEQYALSSGKHPQPFTLKNIENFRRQLKSLGFS-FDYEKEVNTTDPSYYRWTQWIFKQIYKKGLAEIRE-VDVNWCPGLG-TV--LANEEIVENDKGEMVSERGSFPVYKKPMKQWVLKITNYADRLLEDLNLLDWPDSLKKLQTNWIGKEEIDGKITYKLQDWIFARQRYWGEPFPVYFDEDNNVYLIDELVELPHMENIMPSGTGEGPLATNTEWVQYKKNNKIFKRDTNTMPQWAGSCWYYLAYIMKQEDGTYLPIDSKKAYEAFSKWLPVDLYIGGQEHAVLHLLYARFWHKILYDLKIVPTKEPFQKLINQGMILGKDGQKMSKSLGNVVNPDEIIQNFGADTLRVYEMFMGPLTDTKKWNESTVEATYKWILRVKRIFQIFIEDKSKINSLHKDDQFISEHNLLIKEITQDIE-DLKFNIMISKLMIFVNS----------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZIU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1753 14151 8.07 40.31 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 8.07 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_3ZIU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZIU-query.scw PDB file : Tito_Scwrl_3ZIU.pdb: