Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------SSGS--VGSTSPERPVSPGSFDEHEGDQRPCKVTI--EMGVSPGGLFRQLYG----SDYATRGGTVNINQYQCTVDVMCKNPSCPGLPARWEVTSFDKTKQRETHNQPAGGSVWAVGKKHDL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 1E5Q Chain:A ((2-450)) ATKSVLMLGSGFVTRPTLDVLTDSGIKVTVACRTLESAKKLSAGVQHSTPISLDVNDDAALDAEVAKHDLVISLIPYTFHATVIKSAIRQKKHVVTTS----YVSPAMMELDQAAKDA-GITVMNEIGLDPG--IDHLYAIKTIEEVHAAGG--KIKTFLSYCGGL-PAPESSDNPLGY-KFSWSSRGVLLALRNAA--SFYKDGKVTNVAGPELMATAKPYFIYPGFAFVAYPNRDSTPYKERYQIPEADNIVRGTLRYQGFPQFIKVLVDIGFLSDEEQPFLKEAIPWKEATQKIVKASSASEQDIVSTIVSNATFESTEEQKRIVAGLKWLGIFSDKKITPRGNALDTLCATLEEKMQFEEGERDLVMLQHKFEIENKDGSRETRTSSLCEYGAPIGSGGYSAMAKLVGVPCAVAVKFVLDGTISDRGVLAPMNSKINDPLMKELKEKYGIECKEKVVA

General information: TITO was launched using: RESULT: Template: 1E5Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 483 18359 38.01 181.77 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 38.01 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_1E5Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E5Q-query.scw PDB file : Tito_Scwrl_1E5Q.pdb: