Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGCTVKPQLLLLALVLHPWNPCLGADSEKPSSIPTDKLLVITVATKESDGFHRFMQSAKYFNYTVKVLGQGEEWRGGDGINSIGGGQKVRLMKEVMEHYADQDDLVVMFTECFDVIFAGGPEEVLKKFQKANHKVVFAADGILWPDKRLADKYPVVHIGKRYLNSGGFIGYAPYVNRIVQQWNLQDNDDDQLFYTKVYIDPLKREAINITLDHKCKIFQTLNGAVDEVVLKFENGKARAKNTFYETLPVAINGNGPTKILLNYFGNYVPNSWTQDNGCTLCEFDTVDLSAVDVHPNVSIGVFIEQPTPFLPRFLDILLTLDYPKEALKLFIHNKEVYHEKDIKVFFDKAKHEIKTIKIVGPEENLSQAEARNMGMDFCRQDEKCDYYFSVDADVVLTNPRTLKILIEQNRKIIAPLVTRHGKLWSNFWGALSPDGYYARSEDYVDIVQGNRVGVWNVPYMANVYLIKGKTLRSEMNERNYFVR----DKLDPDMALCRNAREMGVFMYISNRHEFGRLLSTANYNTSHYNNDLWQIFENPVDWKEKYINRDYSKIFTENIVEQPCPDVFWFPIFSEKACDELVEEMEHYGKWSGGKHHDSRISGGYENVPTDDIHM-KQVDLENVWLHFIREFIAPVTLKVFAGYYTKGFALLNFVVKYSPERQRSLRPHHDASTFTINIALNNVGEDFQGGGCKFLRYNCSIESPRKGWSFMHPGRLTHLHEGL-PVKNGTRYIAVSFIDP 3EN9 Chain:A ((3-539)) ------MDPMICLGLE------------------GTAEKTGVGIVTSDGE---------VLFNKTIMYKPPKQGINPREAADHHAETFP-KLIKEAFEVVDKNEIDLIAFSQ------GPGLGPSLRVTATVARTLSLTLKK-------------------------PIIG----VNHCIAHIEIGKLTTEAEDPLTLYVSGGNTQVIAYVSKKYRVFGETLDIAVGNCLDQFARYVNLPHPGGPYIEELARKGKKLVDLPYTVKGMDIA-------------FSGLLTAAMRAYDAGE-------------RLEDICYSLQEYAFSMLTEITERALAHTNK------------GEVMLVG--GVAANNRLREMLKAMCEGQ-----------NVDFYVPP--KEFCGDNGAMIAWLGLLMHKNGRWM------------SLDETKIIPNYRTDMVEVNWIAEADIKRDSYLDFDVIIKERVKKGYRDERLDENIRKSRTAREARYLALVKDFG----IPAPYIFDVDLDNKRIMMSYING--------------------------------KLAKDVIEDNLDIAYKIGEIVGKLHKNDVIHN---DLTTSNFIFDKDLYIIDFGLGKISNL-----------DEDKAVDLIVFKKAVLST-------HHEKFDEIWERFLEGYKSVY--------------------------DRWEIILELMKDVERRARYV-------

General information: TITO was launched using: RESULT: Template: 3EN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3050 -135065 -44.28 -263.28 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -44.28 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_3EN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EN9-query.scw PDB file : Tito_Scwrl_3EN9.pdb: