Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDIIGV-KPAEEYIFTIFAMPVGNYFKGGLV-PTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFFGITADN----EFVT-PLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDRFVEAG------ACGTAAVISPIGGIQHGD-DFHVFYSETEVGP-VTRKLYNELTGIQFGDIEAPEGWIVKVD 5BWR Chain:B ((30-369)) -------EPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVP-DAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILC-PVGA----GSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDH-QLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHI--PTMENGPELILRFQKELKEIQYG-IRAHE-WMFPV-

General information: TITO was launched using: RESULT: Template: 5BWR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1673 -113746 -67.99 -354.35 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -67.99 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.373

(partial model without unconserved sides chains): PDB file : Tito_5BWR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BWR-query.scw PDB file : Tito_Scwrl_5BWR.pdb: