Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIVSVMPRSLEEAQA-LD-ATRYLDADIIEWRADYLPKEA----ILQVAPAIFEK-FAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQ-PDYIDFEYYSYKDVFEEML-----DFPNLVLSYHNFQETP--ENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNYTRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKIREILDEA 4CNN Chain:B ((16-252)) PKIIVSLMGRDINSVKAEALAYREA-TFDILEWRVDHFMDIASTQSVLTAARVI-RDAMPDIPLLFTFRSAKEGGEQTITTQHYLTLNRAAI-DSGLVDMIDLELFTGDADVKATVDYAHAHNVYVVMSNHDFHQTPSAEEMVLRLRKMQALGADIPKIAVMPQSKHDVLTLLTATLEMQQHYADRPVITMSMAKEGVISRLAGEVFGSAATFGAVK----PGQIAVNDLRSVLMILHNA

General information: TITO was launched using: RESULT: Template: 4CNN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1189 -7188 -6.05 -32.97 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -6.05 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_4CNN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CNN-query.scw PDB file : Tito_Scwrl_4CNN.pdb: