Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNSEFQPNWLYPPSEVIKNFIQLNNIDTSAF----------DVSTKKIIDDLVNQNTKID--LYTSEVLKEAIGGTTQFWLNIQNQFTINSLRI------QNSSVDNNFSEYRNLVNELKKLHWTPESKYD-YLDQINLKSFFGISEFSLLTKKYIVQHNLRVKFKNIGQYNSSELNVATLIRKAELEAKKQPILSPWNKLLFIEKLQEIKKLSKVKGFKNFRNELLSICNQ----------------------CGVAFIVLDTLNKSPIRGISKFL---DSNIGL--------IIVTTKYNKDDVFWQTFFHEAGHLVLHSKEMVFSD-QGDLNKEASHNLEEAQADEFMLTHILHPYTIEEVTNMFNTRIMYKSKTESWRNICQIARQVNISPSL-----LTGILKFQGIIPYSYFTNGHEKLYD------------------------ 4MT0 Chain:A ((1-445)) ---CTMIPQYEQPKVEVAETFQNDTSVSSIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTLAANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDAAHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYNAVRIA-VQGRRDFRRRPAPAEALIESAKADYAHAA-RSREQARNALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSEVL--LDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWAFAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLADLYKALGGGLKRDTQT

General information: TITO was launched using: RESULT: Template: 4MT0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1233 30226 24.51 83.27 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 24.51 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.140

(partial model without unconserved sides chains): PDB file : Tito_4MT0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MT0-query.scw PDB file : Tito_Scwrl_4MT0.pdb: