Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATPFWYNTALHLLKPFYRWRIKRRAESLELYQQECLERFGPFEAPKNVKAIWFHAVSVGETNAAQPLIEYYLKL-GQPVLVTNTTKTGQARAKSLFLKEPYLDLFQAVYLPV------------DQKPLLKKFFELYQPKLLALVETELWPNLIDQAKLQHVPCLLLNARLSEKSAKGYGKVSGLTAGMLKQLDWVLAQDSATRQRYVELGLDEHKSQVVGNIKFDIHAPEAFIKQAAQLRQQWYLE-N-RQVVTIASTHA-PEEQQILEAFAPYLNSDRELVCIVVPRHPERFDEVFEICQNL-NLITHRRSMGQSIHASTQVYLADS--MGELWLWYALSQVCFVGGSLNEPGGGHNILEPMVLNVPTVVGPRY-FNFQTIVDEFIDENAVLIAQDAQQVVDIWLACLAEPEATEQLVAQAHKVLQRNQGSLQKHIGVINRYLAEKS 4HWG Chain:A ((9-376)) ------------------------------------------------MLKVMTIVGTRPELIKLCCVISEFDKHTKHILVHTGQNYAY-ELNQVFFD-----MGIRKPDYFLEVAADNTAKSIGLVIEKVDEVLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNRCFDQRVP--EEINRKIIDHISDVNITLTEHARRYLIAEGLPAELTFKSGSHMPEVLDRFMPKILKSDILDKLSLTPKQYFLISSHRE--KNNLKELLNSLQMLIKEY-NFLIIFSTHPRTK-KRLE-DLEGFKELG-------------DKIRFLPAFSFTDYVKLQMNAFCILSDS-------GTITEEASILNLPAL-NIREAHERPEGMDA--GTLIMSGF-KAERVLQAVKTITEEHDNNKRTQGLVPDYNEA-GLVSKKILRIVLSYVDY--

General information: TITO was launched using: RESULT: Template: 4HWG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1821 -673 -0.37 -1.97 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -0.37 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_4HWG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HWG-query.scw PDB file : Tito_Scwrl_4HWG.pdb: