Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSSNTSRIAVQQIDPHELKAWIKAQALDLGFADCVSAKPDAQEQMPRFLEYLERGYHADMTYLEENLEKRADPTLLVPGTKSIICVRMNYLVESPKPRYVPFEPNSA-ITARYARGRDYHKVMRGRLKTLATR-IREKVGDFESRPFADSAPIFEKSLAESAGMGWTGKHTLLIHKKSGSLFVLGELFTSLDLPFDEPATSHCGSCSACIDICPTQ---AIVEPYTLDARRCIAYLTIEYKGIIAEELRAGIGNRIFGCDDCQLICPWNSFAKTASIPDFNPRHGLDNISLLDIWQWDEATFLANTEGSPIRRTGYQSFKRNIAIGLGNAPYSKEIVDQLNKGKSLHDEIVNVHID---WAIEQQLNQL- 4MFL Chain:A ((23-345)) M-DPETVRIALGL--EERTAAWLTELDELGPPAEPVRLPRG--EEARDLLRRLEVPELDAEEIVAAAPDPDRDPALWWLLERTHHAIVRHMGDHRAKPRGGPPLPYEGGAAARYFHVYVFLATVPAVRRFHAERGIPDEVGWETLTQLGELVAIHRRKY----GQGGMNMQWWTTYHLRGILYRLGRLQFSLATGKDG--TPHLGLA------VPEWGGPLLPKAYDESLHRARPFFDRHFPEHGAR------------------VAWGSSWMLDPQLEEYLTEDS-------NIIQLARFWTLTDSAPEPGNADGDSSILEFVF------RYNGQPLDELPQRSSLERAVIAHLKAGRHWHMRTGFVKLP

General information: TITO was launched using: RESULT: Template: 4MFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1450 -53409 -36.83 -170.09 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -36.83 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_4MFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MFL-query.scw PDB file : Tito_Scwrl_4MFL.pdb: