Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRFAINGYGEAQDVFEEI-DAHPR-EVGSGHVRVEIKAFSVNPYDVALRLGEMKEI-------------RTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHA-------------------VGGTYGEEVVLPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLIQLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFA-----DQADLVIDATKGSIKGETGIQIMKPGGRYVALNDLPDL--DLRQK---KEGFYESFVPRKEYLDAEAFAGIIKAYQKGAFHVFISMNLSASLKHVIQAHQLVEG-HPPAGKIILSFEK 4J6F Chain:B ((34-382)) MTAVLLTGHGGL-EKLVYSRDV-PVPAPAAGEVLIKVTACGMNNTDVWVREGAYGTEDDPSAVSTWRRHGNTLTFPRIQGTDTVGHIVAVGEGVDRARIGERVMVDFSIYNRDDDSLADIDYMGHGRDGGYAEYMALPAENAHVVATDLTDIELATFCCAYLTGERMLER-ARLAAGETVLVTGASGGVGSAIIQLARARGAVPIAVAGPGKEAAMLDIGAQAVVTRGQGDLAEAVEAASGGRPIDVVADLVGGPL-FNDLLKILRPEGRYTTAGAIAGPVVQLDLRTMYLKQLELH---GSSQGSRADFRRLVRYIEEKKIRPLVGGVY--PLSEFHRAQTDFMAKN-FVGKLVVVPD-

General information: TITO was launched using: RESULT: Template: 4J6F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1710 17261 10.09 56.78 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : 10.09 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_4J6F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J6F-query.scw PDB file : Tito_Scwrl_4J6F.pdb: