Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MYKMTRRSTML--ETYLEKSIFR--QVYICEQLHEKGTIQIR-EIADQLNVC---PLTVTNDLECITDILHQ-------QIISVEKSRSSFTVAFDSSFSRLALTQL----IYQRSDFFNVLGH-------YLSGEHNWS-NIA---------------DTTF--------------ISLSKVYNLRNELINFFKQMQYLNADGHFEIPEK--DYRSLLLTIIYETNRTELIQ------LNKSIIQSG-------------QQLI--DYVE----KHFFS-------------RSYPTVEKQFIL---------LGIAIGLQRSKTNPIYFSQEEK------------RVARHTPLFQL------------IQKGLEQLSFSLCHDEDECLYIYFLFNSRNYLCDNFELLQKD-------------LNVVYQNHVADNPLVIELSQKLILS-----LNISEENQILFDKALLPFIRSLWADIQ---IFQPDKTYLLN-EEQRALYLEVHEI---------------LEQWRKQNNLFLRWNENSIRKLTISLS-LLNEHKRKSPIEVFIVAPSDFRY----LYYRQQLEDILG----EHFSI--SNI------ICKQLREIVDDTFFCTQRIILCDSSLYQEG-------------LGSEKTIIYPITFQTIHTVIDQLNKQI------------------------------------- 5GKD Chain:A ((1-726)) ADLLVKTPEAYDQALKKAKPGDDIILANGTWRDFEVLFEAKGNENKPITLRGQTPGKVFLTGQSNLRLAGEHLIVSGLVFKDGYTPTGEVIAFRRNKD--VLASHSRVTQVVIDNFSNPEKFEQDSWVMVYGRHNRFDHNHLVGKRNKGVTMAVRLTTESSQQNHHRIDHNYFGPRPILGSNGGETLRIGTSHHSLTDSFTLVENNYFDRCNGEVEIISNKSGKNSIRNNVFFESRGTLTLRHGNGNIVENNVFFGNGVDHTGGIRVINRDQIIRNNYLEGLTGYRFGSGLTVMNGVPNSKINRYHQVDNALIENNTLVNVEHIQFAAGSDKERSAAPINSNMNNNLIVNDQGTDGITAFDDISGIKFKDNLLNQDAKPSINKGFEQADITMQRHDNGLLYPEAKTQQKYGVSTQLEPIGKDEVGVSWYPKVEPDVAFGSGKHIAVSPGDNTLFDAIASAETGDVLVLQAGEYWVSKILSLDKTLTIRAQEKGSAVIFPQRSTLIEINNKGNLTLDGVYVDATNAPDAAGNTLIRTTRLPMQRNYRLAIKNSTFENLDINHSYHFFDAGNRSFADYIEVQDSQFKHITGDLFRLNKETDDLGIYNVEYLTIENSNVSDLQGAIAKVYRGGTDESTFGP-HVVMNNNIFNEVGKGKRNKSAASLILHGTQVNKMTTNEFNNSAPIIFELTVGEPKTWVTGNVFEGTPEPVVRDLFPLSGATTTISGNTVL

General information: TITO was launched using: RESULT: Template: 5GKD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3104 57648 18.57 119.11 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 18.57 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.071

(partial model without unconserved sides chains): PDB file : Tito_5GKD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GKD-query.scw PDB file : Tito_Scwrl_5GKD.pdb: