Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MISKRKHVIIFPILCKKIQGGGSTMIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEI---DRYSPRFTEMER-RLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSY-QLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI---ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK 5F4H Chain:A ((1-437)) MNDLMLD--KSALLFG-------------VSKYLEKGIIT-----GNVLIH-----KSLLAELERESNDGLVSAEIALDEVKKLKDITERILVNFEIVGDDS--KKGEANELSREYCLEKGCIIVTADETQKKICDAMGIQYNFLQPLKQGLSFESFFDDETMSLHIKEDTVPRAKKGKPGNWKFVNLS------DKPMLSTDVRMIANEIINAVRLIKGSFVEIERRG--SLIIQLGNYRVVITRPPLSDGWEITITRPVVRKRLEDYNLDERLIKRLEERAEGIIIAGAP--GMGATTFAQALAEYYMRLGKI-----------VKTIESPRDMHLPPE---------ITQYSKNYAEIGELHDILLLSRPDYTVYDEMRN---DEDFKLYVDLRLAGVGMVGVVHATSPIDAIHRFVNRVDIGTIPNILDTIIFINSGNVSKVYTLEMTVKVP--------AGLKEADLARPVVEIKDLATGNTEYEIYVFG-----EQTMIVPVNR-

General information: TITO was launched using: RESULT: Template: 5F4H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2113 15039 7.12 35.38 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 7.12 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.137

(partial model without unconserved sides chains): PDB file : Tito_5F4H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F4H-query.scw PDB file : Tito_Scwrl_5F4H.pdb: