Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDVIVIGAGPSGMTAALYASRSNLSVL----MIEQG-APGGQMNNTAEIENYPGF-DSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICE-DKSYEGKT---VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGA--FFRNKRLVVIGGGDSAVEEAIYLTQFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVKTGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPT-DNEMKTNMPGVFAIGDVREKDLRQITTAVGDGGIAGQQVYKYLESLEETTA 3ITJ Chain:D ((25-337)) ---VTIIGSGPAAHTAAIYLARAEIKPILYEGMMANGIAAGGQLTTTTEIENFPGFPDGLTGSELMDRMREQSTKFGTEIITETVSKVDLSSKPFKLWTEFNEDAEPVTTDAIILATGASAKRMHLPGEETYWQKGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKVFMLVRKDHLRA------RAEKNEKIEILYNTVALEAKG----LNALRIKNTKKNEETDLPVSGLFYAIGHTPATKIVAGQVDTDEAGYIKTVPGSSLTSVPGFFAAGDVQDSKYRQAITSAGSGCMAALDAEKYLTSL-----

General information: TITO was launched using: RESULT: Template: 3ITJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1746 59952 34.34 206.73 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : 34.34 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_3ITJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ITJ-query.scw PDB file : Tito_Scwrl_3ITJ.pdb: