Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNVAPLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTR-SDGILALGGGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPAGVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKD--LLEHAVPIITATLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHAEKIGLSPTGSTAALERILQKFHLPITYDL-SHPDELFHAITHDKKARGSQLNIILLEKIGQAKIVPVPTETFKDYLGGL 5HVN Chain:A ((15-361)) -------SPSYNIIVDSVL--DFSHILEYV-TNKQVLVVTNTTVAKLYLTKFLAALVD-DLDVRTCILEDGEQYKSQQSLDKILSTLLENHFTRNSTVLVALGGGVIGDITGFAAAIYQRGIDFIQIPTTLLSQVDSSVGGKTAINHQLGKNMIGAFYQPKVVYTSIEFYKTLPQREYIAGMAEVVKYAFISK-DFYLWLDSNRDKILAKDSVTLIEMVKRSCQIKAQVVAMD---TGARAILNFGHTFGHAIEKCQNYRGLKHGEAVGVGMAQAIDFSHYLGLISQQQAKDFNDFIVSFGISIDFPNDICQKEFLEAMLLD----NKELKFILIENIGSLSLQKQSKNELEQFLDIS

General information: TITO was launched using: RESULT: Template: 5HVN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1806 -80638 -44.65 -240.71 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -44.65 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_5HVN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HVN-query.scw PDB file : Tito_Scwrl_5HVN.pdb: