Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPELPEVETVRKGLERLVVGKTIQKVQVLWPKII----EQPETPIFEASLVGETIQSIGRRGKFLIFHL-DHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDGSQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKPLLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGGTTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKIAK 1XC8 Chain:A ((1-270)) -PELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQ-----LKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADG-QLIYADVRKFGTWELISTDQVLPYFLKKKIGPEPTYEDFDEKLFREKLRKSTKKIKPYLLEQTLVAGLGNIYVDEVLWLAKIHPEKETNQLIESSIHLLHDSIIEILQKAIKLGGSSIRTY-SALGSTGKMQNELQVYGKTGEKCSRCGAEIQKIKVAGRGTHFCPVCQQ-----

General information: TITO was launched using: RESULT: Template: 1XC8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1361 45164 33.18 170.43 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 33.18 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_1XC8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1XC8-query.scw PDB file : Tito_Scwrl_1XC8.pdb: