Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDTDENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVMCNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLSQEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAKKGNMVTL--DGEVKGQHAGLMY-YTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQGFHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFDEPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV 5MKO Chain:A ((49-280)) ----KDERILVAVSGGKDSAVTAYVLKKLGYNIECLHINLGISGY------SEKSEEYAKKQCKLIGAPLHIVRIKEILGYG-----IGEVK--TRRPPCSYCGL-TKRYIMNKFAYDNGFDAIATGHNLDDEAS-FLLNNILHWNTEYLAKGGPILPQ-QGKFIKKVKPLYEVTEREVVAYALAVGLEYIVEECPYARGATTL-DMKGVLNELEEKRPGTKFNFVRGYLKKKKLFEPEIKEKEIKECKICRM---------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5MKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 991 -73409 -74.08 -320.56 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -74.08 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_5MKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MKO-query.scw PDB file : Tito_Scwrl_5MKO.pdb: