Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIELNQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVEDKEMPHLRKGTIIKIDQEDQNGGKLYGNHA 1M5I Chain:A ((130-239)) ---------STG-YLEELEKERSLLLADLDKEEKEKDWYYA-QLQNLTKRIDSLPSLQTDMTRRQLEYEARQIRVAMEEQL---------------GTC-QDMEKRAQRRIARIQQIEKDILRIRQLLQSQA

General information: TITO was launched using: RESULT: Template: 1M5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 175 -8064 -46.08 -76.80 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -46.08 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.143

(partial model without unconserved sides chains): PDB file : Tito_1M5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M5I-query.scw PDB file : Tito_Scwrl_1M5I.pdb: