Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFTTEIVQTLV--TKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHH-----YTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE 5HJQ Chain:A ((78-367)) ALRSYTEIQQLLQQGKKRDVKNILRENSWP-INSPIRAQLWPMLCGQHLD-----------GFYWEMVHQVFGTTELS--------EKPIMLPAF------VDATHCLPYHLTSTGRAVADRIVNVLGYDCPDITYSP-VLYPITSILLHFMS---------------EEEAYICLAGLVGSKEKVFINQTKLQHEVTWKTVMQIAKKHTKSATSYFQRICPGLKLERIFM----------DWCWWILAGLPFQHL-VRIMDCYFHE------------------------------GI-------------KVLYRVALVILNLFHKECQSN--------------NEWSPDNIKNDIGNALIKFCKKIPVS-PAKLLHAAFS-IRGLSTQYISR---

General information: TITO was launched using: RESULT: Template: 5HJQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1263 -18134 -14.36 -65.46 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -14.36 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.219

(partial model without unconserved sides chains): PDB file : Tito_5HJQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HJQ-query.scw PDB file : Tito_Scwrl_5HJQ.pdb: