Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNHENYWTFCPHNLFWIDRKINRIDMSQTTQTDIITLGDVATRLPSFIPKVPHILNGLKQAYLRTSNTPTGLGIAFEKAVKRNPKGTALLFEDQSYSYEALNEWANQISHYYLSLGARKGDVIAVMIENRSELIATIVGLAKIGVTIALVNTSQVGKVLAHSINLVKPIAVIAGEEVRAAID-EI----RQDLNVPKDRFHWFADQATRQKSGTAPKGYVNLADQIDQFPKFNPSTTRSVTGNDGLFYIYTSGTTGLPKAVIFKHSRWTLAYGTYGHILNLGPDDVMYVTLPLYHATGVVV-CWCGVIAGSATLAIRRKYSTSAFWKDVQKFNASAIGYVGELCRYLMDAPVTELDRNHRVTKM--IGNGMRPNIWDKFKQRFGVKEVLELYASSEGNVGFSNIFN----FDNTVGFSPTPYAIIQFDKEKNEPIRDKNGWCQKVKAGEVGLLIGKITSRSPF-DGYTDPEKNKSVIWKDVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWKGENVSTTEVENMVCEYDKIAEAVVYGVEIPNTNGRAGMAAITLADGEELNDADLTEMVTVFKKC-LPTYAVPVFLRVQKKVETTGTFKYQKNKLKEEAFNPSKTSERLLALLPGASSYCDITTEIFDNIQAYKYRF 1MDF Chain:A ((24-532)) ---------------------------------------------------------------------GETFGDLLRDRAAKYGDRIAITCGNTHWSYRELDTRADRLAAGFQKLGIQQKDRVVVQLPNIKEFFEVIFALFRLGALPVFALPSHRSSEITYFCEFAEAAAYIIPDAYSGFDYRSLARQVQSKLPTL-KNIIVAGEA----------EEFLPLEDL----HT-EPVKLPEVKSSDVAFLQLSGGSTGLSKLIPRTHDDYIYSLKRSVEVCWLDHSTVYLAALPMAHNYPLSSPGVLGVLYAGGRVVLSPSPSPDDAFPLIEREKVTITALVPPLAMVWMDAASSRRDDLSSLQVLQVGGAKFSAEAARRVKAVFG-CTLQQVFGMAEGLVNYTRLDDPEEIIVNTQGKPMSPYDES--------RVWDD--HDRDVKPGETGHLLT--RGPYTIRGYYKAEEHNAASFTE------DGFYRTGDIVRLTRDGYIVVEGRAKDQINRGGEKVAAEEVENHLLAHPAVHDAAMVSMPDQF-LGE-RSCVFIIPRDEAPKAAE---LKAFLRERGLAAYKIPDRVEFVESFPQTGVGKVSKKALREAISEK-----------------------------------

General information: TITO was launched using: RESULT: Template: 1MDF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2987 -47038 -15.75 -95.03 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -15.75 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_1MDF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MDF-query.scw PDB file : Tito_Scwrl_1MDF.pdb: