Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAYFEKLLDSSQQQKQLPLIFPLLIALFSGAVFSFALAPYYWWWLAILSPALLYATLHNRSAKQAFAIGWSYGFGLWFVGAFWLYTSIHVYGDTNAFLSVCMIAVMALVMGLFTAFQTWVYRRFFPETPLTFAPLWIIFEWAKTWVFTGFPWLFVGYAFTERLLDGYAPLFGIYAISFVVIVLACALVEVLRKRIFWVIPAALLVLGAWGASYIQFVQPKATKPLSVSLIQGNIPQDLKWLTEYQVRTLEIYAGLTQSEWGRDLIVWPESSIPLFQTDI--------EPFLDAMDAQAKKN------HTAWVTGIPYWDVTKS--HQVGSPLYYNSIMASGSD-SSGLYKKQRLVPFGEYIPLSGLLSWVLPAMQNDISMSGFTRGESDQKPLLI-KGHALAAAICYEVAYPNLTR-RN-AEDSDFLVTVSNDAWFTGTAGPWQHLQMVQMRAKENGRWFIRATN---------TGVTAFIDQNGHITEQAPIDKEFVLRGDLPAMQGQTFYNRLGDYPILGFAVLLLVLGWIYRPRKVDVSYKSRR 3N05 Chain:A ((3-277)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LQLRLALNQIDSTVGDI--AGNAEAIL-RWTRHSAEQ-GAHLVAFPEMALTGYPVEDLALRSSFVEASRTALRELAARLAEEGFGELPVLVGYLDRSESAQPKYGQPAGAPRNAAAVLHRGRVALTFAKHHLPNYGVFDE-----------------FRYFVPGDTM--PIVRLHGVDIALAICEDLWQDGGRVPAARSAGAGLLLSVNASPYERD--KDDTRLELVRKRAQEAGCTTAYLAMIGGQDELVFDGDSIVVDRDGEVVARAPQFSEGCVVLDLDLP-----------------------------------------

General information: TITO was launched using: RESULT: Template: 3N05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1280 -15529 -12.13 -67.52 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.48 3D Compatibility (PKB) : -12.13 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_3N05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N05-query.scw PDB file : Tito_Scwrl_3N05.pdb: