Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSTAKHNYAPDILGAGYEQLTLNFPDDYEGKVVATLVRKKATQPTQKAVLYIHGFLDYF-FQTEMAEQFNAHGYDFYALDLRKYG-----RSKLPHQIFYNVLDLIEYDAEITQALEIIGQEKHTQVLLAGHSTGGLTATLYAAHHPDHPLIKALWANSPFYDFNLSLVEKKFGIPMLSRVGKYLPKVKFPSQLNKWYTTSLHKQLKGEWDFNLDWKPTSAPTVQ-LSFVHAIHTAQKEIHRG----VKLNI----PALIMHSHQTKNPRKWGPDATQSDVILDVKDIAKFGKKMKGDVSVVSIHNGLHDLVLSAQPVR--EQVYQQLFQWLDQKVT-- 4ZWN Chain:A ((22-331)) ------SAPYPYKVQTTVPELQYENFDGAKFGYMFWPV--QNGTNEVRGRVLLIHGFGEYTKIQFRLMDHLSLNGYESFTFDQRGAGVTSPGRSKGVTDEYHVFNDLEHFVEKNLSECKAKG----IPLFMWGHSMGGGICLNYACQGKHKNEISGYIGSGPLIILHPHTMYNKPTQIIAPLLAKFSPRVRIDTGLDLKGITS-----DKAYRAFLGSDPMSVPLYGSFRQIHDFMQRGAKLYKNENNYIQKNFAKDKPVIIMHG--------------QDDTINDPKGSEKFIRDCPSADKELKLYPGARHSIFSLETDKVFNTVFNDMKQWLDKHTTTE

General information: TITO was launched using: RESULT: Template: 4ZWN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1682 10331 6.14 35.50 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 6.14 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_4ZWN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZWN-query.scw PDB file : Tito_Scwrl_4ZWN.pdb: