Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKQTVQLEIISVNQTKTKIELVISQIEQQIKNRSLTPGTRLPSVRKLAKDLGFSVSTIVEAYERLIALGKIESRSGSGFYVVAPLAPLALNELGPKLDRSIDPLWISRQSLEAEPNAFKPGCGWLPNDWMPLESIRKALRSATRSEDDSLLGYSSPLGLSALRDLLARRAQS-KGIE-ANLNQVLLTDSGTQAIDLVCRFLLKPDDVVLIDDPCYFNFHALLKVHQVKVVGIPYTPNGPDLEAFKEAIEG-------YNPRLYITNSGIHNPTGATLSLSTAHQLLKLIDQSNLIVIEDDIFSDFEYTP--APRLAALDNLSRVIFIGSFSKTLSASIRCGYIIAKPEWIDQIADLKIATSFSHNGLSAKILHTALTDGSYRKHLDLLKVRLAQAMQETIAKLKSI-----RIEPWIEPKAGIFVWCRLPEGVDAEKIAQFCLNRQVILAPGNAFSQAQSAGQFIRFNITQSNHDYIY---KTLAEALLQESLEKQV 1VP4 Chain:B ((41-422)) -----------------------------------------------------------------------------------------------------------------ADKDAISFGGGVPDPETFPRKELAEIAKEIIEKEYHYTLQYSTTEGDPVLKQQILKLLERMYGITGLDEDNLIFTVGSQQALDLIGKLFLDDESYCVLDDPAYLGAINAFRQYLANFVVVPLEDDGMDLNVLERKLSEFDKNGKIKQVKFIYVVSNFHNPAGVTTSLEKRKALVEIAEKYDLFIVEDDPYGALRYEGETVDPIFKIGGPERVVLLNTFSKVLAPGLRIGMVAGSKEFIRKIVQAKQSADLCSPAITHRLAARYLERYDLLEQLKPTIELYRRKRTVMLNALEEYFSDIPGV-KWVKSEGGLFIWLTLPEGFDTWEMFEYAKRKKVFYVPGRVFKVYDEPSPSMRLSFCLPPDEKIVEGIKRLREVVLEYGKEK--

General information: TITO was launched using: RESULT: Template: 1VP4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2006 -20380 -10.16 -56.14 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -10.16 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_1VP4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VP4-query.scw PDB file : Tito_Scwrl_1VP4.pdb: