Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMLIRKAKKDDTDTIVAIIAPYVDQVIANDEGRSRFKSEAIQTIFDREDIHYFVGEIDQQIVGIVAYMEPS------HLMHFFIKKTHLKLGLGRQLWDFIEEKIKNENIDIEKITVN-SSFYAQDIYEKFGFIVSGDAAEKWGIRFIPMTKYYALASEK------- 1MK4 Chain:A ((0-156)) -HMDIRTITSSDYEMVTSVLNEW--------WGGRQLKEKLPRLFFEHFQDTSFITSEHNSMTGFLIGFQSQSDPETAYIHFSGVHPDFRKMQIGKQLYDVFIETVKQRGCTRVKCVTSPVNKVSIAYHTKLGFDIEKGTKTVNGISVFA--NYDGPGQDRVLFVKNI

General information: TITO was launched using: RESULT: Template: 1MK4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 558 -24816 -44.47 -173.54 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -44.47 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.210

(partial model without unconserved sides chains): PDB file : Tito_1MK4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MK4-query.scw PDB file : Tito_Scwrl_1MK4.pdb: