Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIFLPGASGSTTFWHPLIEKLPQQYRAKIIGYPSFGDTPESLEVKSFEDLTNYVVNQIHDESVIIAQSMGGIFAVTAALQKPQLVKGLVLIATSGGINLEP----FN---V--------QDWRE---AYRQA-------FL----KYP-----------DWFI-----TTNANYEEFLSDINIKTLLIWGDNDPVSPVQVGQYLNQKFENSTLYVVKGGDHQLAEKYADEVAVQIKNYLKGLM 4NMW Chain:A ((17-258)) CHLVLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGF-GAMTLEEMTAQVAKNAPDQAIWLGWSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVEVLNGGLEILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIVPLLDTLWPHSTSQIMAKAAHAPFISHPAAFCQALMTLKSS--

General information: TITO was launched using: RESULT: Template: 4NMW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 991 -81637 -82.38 -414.40 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -82.38 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4NMW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NMW-query.scw PDB file : Tito_Scwrl_4NMW.pdb: