Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKFVMMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRAKSLNELEHAASLIVWQAHEEGPM-------SELIWAPEVHYINGKWYIYFAASNHKTIRDESHHHRMFVLENQHIDPMNTD---WIEKGQIKTK----RETFSLDGTTFSS-QGKLYYVWAQQDPEITGNSNLYLSEMDNPW-TLKGK--QILLSIPE--YEWE--KRGFSVNEGPAVLTRNGKIFITYSGSAT----DENYAMGLLWADEHKNLLLKESWHKL-SEPVFVSS------EKNKQFGPGHNSFTVSEDGCFDILIYHARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL 1GYH Chain:E ((6-310)) -----------------DVHDPVMTRE-GDTWYLFSTG---PGITIYSSKDRVNWRYSDRAFAT--E-PTWAKRVSPSFDGHLWAPDIYQHKGLFYLYYSVSAFG-----KNTSAIGVTVNKTLNPA-SPDYRWEDKGIVIESVPQRDLWNAIAPAIIADDHGQVWMSFGSFW------GGLKLFKLNDDLTRPAEPQEWHSIAKLERSVLMDDSQAGSAQIEAPFILRKGDYYYLFASWGLCCRKGDSTYHLVVGRSK---QVTGP--YLDKTGRDMNQGGGSLLIKGNKRWVGLGHNSAYTW-DG-KDYLVLHAYEAAD-------NYLQKLKILNLHWDGEGWPQVDEKELD--

General information: TITO was launched using: RESULT: Template: 1GYH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1547 30688 19.84 112.82 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain E : 0.67 3D Compatibility (PKB) : 19.84 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_1GYH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GYH-query.scw PDB file : Tito_Scwrl_1GYH.pdb: