Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVIEDAAESLGSTYKGISSGLF-GTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQARDQAPY-YQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRIEMMPEVD-GMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK 1MDO Chain:A ((9-383)) ---------------DFLPFSRPAMGAEELAAVKTVLDSGWI-TTGPKNQELEAAFCRLTGNQYAV-AVSSATAGMHIALMALGIGEGDEVITPSMTWVSTLNMIVLLGANPVMVDVDRDTLMVTPEHIEAAIT------PQTKAIIPVHYAGAPADLDAIYALGERYGIPVIEDAAHATGTSYKGRHIGARGTAIFSFHA--IKNITCAEGGIVVTDNPQFADKLRSLKFHGLGVD--QAEVLAPGYKYNLPDLNAAIALAQLQKLDALNARRAAIAAQYHQAMADLP-FQPLSLPSWEHIHAWHLFIIRVDEARCGITRDALMASLKTKGIGTGLHFRAAHTQKYYRER---FPTLTLPDTEWNSERICSLPLFPDMTESDFDRVITALHQIA-

General information: TITO was launched using: RESULT: Template: 1MDO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2130 -106797 -50.14 -295.84 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -50.14 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_1MDO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MDO-query.scw PDB file : Tito_Scwrl_1MDO.pdb: