Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGDELIISHGNGPQVGNLLIQQQ-AADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEKGIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGWRKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPV-VETATGLEGREAVIDKDFASEKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPKVEAAIAFVEAKPNAKAIITSLENIENLLAS-EEGTIIVAD 2WE5 Chain:A ((2-310)) --GKKMVVALGGNAILSNDASAHAQQQALVQTSAYLVHLIKQGHRLIVSHGNGPQVGNLLLQQQAADS-EKNPAMPLDTCVAMTQGSIGYWLSNALNQELNKAGIKKQVATVLTQVVVDPADEAFKNPTKPIGPFLTE-AEAKEA-MQAGAIFKEDAGRGWRKVVPSPKPIDIHEAETINTLIKNDIITISCGGGGIPVVGQEL---KGVEAVIDKDFASEKLAELVDADALVILTGVDYVCINYGKPDEKQLTNVTVAELEEYKQAGHFAPGSMLPKIEAAIQFVESQPNKQAIITSLENLGSMSGDEIVGTVVTK-

General information: TITO was launched using: RESULT: Template: 2WE5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1628 -20763 -12.75 -67.85 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -12.75 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_2WE5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WE5-query.scw PDB file : Tito_Scwrl_2WE5.pdb: