Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WNDGGFVNKQQAHDLLI-NKPDGTFLLRFSDSEIGGITIAWKFDSPE--RNLW-N-LKPFTT------RDFSIRSLADRLGDLSYLIYVF 2MK2 Chain:A ((16-95)) ----RDLSRAAAEELLARAGRDGSFLVRDSESVAGAFALCVLYQKHVHTYRILPDGEDFLAVQTSQGVPVRRFQTLGELIGLYA------

General information: TITO was launched using: RESULT: Template: 2MK2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 224 -17784 -79.39 -257.73 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -79.39 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_2MK2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MK2-query.scw PDB file : Tito_Scwrl_2MK2.pdb: