Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYSLARPMLFSLAPERAHEL-----TLSMLDKAHKLGIMRQTVEAKPTTCMGIEFPNPVGLAAGLDKNGAHIDALAGLGFGFIEIGTITPHPQSGNPKPRLFRIPEAKAIINRMGFNNDGVDKLIENVKASKFRGI--------LGINIGKNADTPVEKAVDDYLICLEKVYNYASYITVNISSPNTKNLRSLQSGDALTELLQALKARQLELAEQYNHYVPLVLKVAPDLTAEDVEFISAQLLDFKIDGLIVTNTTLSREGVENLPYGNESGGLSGAPVFEKSTECLRLFAQTLKGQISLIGVGGILSGEQAAAKQQAGATLVQIYSGLIYTGPTL---VKQCVEAMT 2FPT Chain:A ((49-376)) ------------LDPESAHRLAVRFTSLGLLP---F-----QDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNK-TSVD-AAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLT--KVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEAL-

General information: TITO was launched using: RESULT: Template: 2FPT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1832 64474 35.19 208.65 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 35.19 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_2FPT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FPT-query.scw PDB file : Tito_Scwrl_2FPT.pdb: