Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNNSQNLIMLKHDFASHIEDLKCATKPRSEALGRHLWTCTID-ADFYWLKFHLPNVHAQSEQDFLHELQFYEDITYKKANWLLPFKIIEGRTISQQTQFQGKVLV--LPDTECWFDDLDQ---KQNLKNINEKIYLALVKLAELHELGWIHGDIKKEHFRKFK-QELYLIDFEKTRLISSP----DPITDATPRYMAPELFHGANKTVQSDLYALGIVLYEWLT-QTRLQANSYHEWAVLHC-QKLNVELPSS-FQIFLPLLSGLLQKQQQNRFSNVHEAINCLKALST 5J87 Chain:B ((8-270)) -------------YGSWEIDPKDLTFLKELGTGQFGV-VKYGKWRGQYDVAIKMIKEGS--MSEDEFIEEAKVMMNLSH--EKLVQLYGVCT--------KQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPT-FKILLSNILDV--

General information: TITO was launched using: RESULT: Template: 5J87.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1230 -10926 -8.88 -43.88 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -8.88 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_5J87.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J87-query.scw PDB file : Tito_Scwrl_5J87.pdb: