Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTFVDAARSFRTQF------DLNFSEKIIVDFFAGGGGA--STGLEMGLNRPVYVAVNHNPKAISMHEANHPHAKHYIQDVFAVDPIDIC-DGHQVGWFHASPDCTHHSQAAGGQ-------PRKKEIRDLSWVVLKFAGKVKPDVISLENVKQILGWGPLIAKRDKATGRVITLDKININGKKVNRIAEPGERVPRHNQFLVPNPKKKGKTWKHFVRS-LEQLGYEVEWQKNIIAADFGAPTKRERLFLIARC----DGQP---IVWPEKYFSKKPKGNLKKWRSTVECVDFSDLGNSIFDRPQGPLADATLKRIAKGIQKYVIETKEPFFVNSSTPFIGRDFRTSFGHDIREPLATTTAGYGGHSSLISPI---LVPFITEFANASQQRNWSIDEPLSTICAQVKGGHHGLVTAKLSKDNYKGAL--RVAAFLINYYGNGDARS-ITEPMDTITTKDRLVLVTVWIKGEPWAIVDICIRMLKPRELFRAQGVPDSYVIEYGSDGKPLSKKDQVFMVGNSVSPYPMAAIARANNPFITQQIKGAA- 5MF1 Chain:A ((4-561)) ----VIASGRLEKCVVDGVTEELDCQEKVVVTLTVGNGQSLQTEALEFSLA-PLRVSLTKSPLWASYPLQYLSSFNWKPLEVILRPSNKVCKDGD----WEDSPTCGWFSQRVADSQGFCCECERTRANLDCDF------------------------WSPVSAHCLTFDPQWYSGYELGAASLQFEIAITVEVREVLHLGPSVPLASSASRLLSAKLLGDLAMYTQLPAISNQVLMVPQPPATNRSAWMLLDKTMLSMDGLACDKVGTGFSAFRYQPSGC---GRAPQACLS----------GQLKDLWEADLARIADG---------------------------------RVPLYMITRFTGGSDTTLQSFSGGPLSFALPVTSHSQ----------SLVTLSVAADGVRLVTNR-SPGKITGAAVCRFAGTSCGGFEAVAARGYIYVNITNTGRLDSDYTLTVSNCSSNVRPIEARTLAVRAGSAASLDPPMELYV----EDQAAAAARTCTVSLYDSV--------GAVTDSLTLSFYTNATQ

General information: TITO was launched using: RESULT: Template: 5MF1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2108 18902 8.97 45.55 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 8.97 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.150

(partial model without unconserved sides chains): PDB file : Tito_5MF1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MF1-query.scw PDB file : Tito_Scwrl_5MF1.pdb: