Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREKMRFPTLVFDIETLTDLKAGAHLYHLDLPEADVEQALTKIRRQESGMDFQRLPLHEIVCISGLWIDESGFRLFS-----FSREHYSEAEILQKFLSIFDKRHP-TLVSWNGSQFDLPVILFRAMYHGLSAPGLFDQGELDSQK-RFNN-YQNRY-HHRHIDLMDVMAMF-NGRNFQKLDDVACILGLPGKRGESGYHVPEYVRTE-QWLKLTSYCEGDVLNTWFIYLRWLLLKGQMNVDEHNHWISSSIEYLQTMPQQADFLEVWQRTSKHTEFTSHYFNPLNF 1TGO Chain:A ((133-328)) ----EELKMLAFDIETLYHEG-------------------------------EEFAEGPILMISYADEEGARVITWKNIDLPYVDVVSTEKEMIKRFLKVVKEKDPDVLITYNGDNFDFAYLKKRSEKLGVKFILGRE--GSEPKIQRMGDRFAVEVKGRIHFDLYPVIRRTINLPT-YTLEAVYEAIFGQPKEKVYAEEIAQAWETGEGLERVARYSMEDAKVTYELGKEFFP-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TGO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 861 -4652 -5.40 -25.15 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -5.40 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_1TGO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TGO-query.scw PDB file : Tito_Scwrl_1TGO.pdb: