Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIYDGQVADNYMYQDSNQAAIVVSHSTPSLPYPFTMKPNNHSTETNTPPAIDVEKFVAKLESITSRRSKARCARSIRIALESAGADVENHPIAASDWGDTLKKIGYKEINPAFDEPQEGDIYIIHRTRNHIYGHIAGYTGSEWVSDFKQSSYDVYKDDNVTYTYYRLG 2L8K Chain:A ((1-123)) -----GLTATLAALTDDDFQFLSDVLDCRAVRSAMNLR--------AALTSFQVAQYRNILNASLQVDRDAARSRRLMAKLADFAVEQEVTA------GDRVVVIDGLDRMAHFKDD-----LVLVPLTTKVVG------------GSRCTICDVVKEE----------

General information: TITO was launched using: RESULT: Template: 2L8K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 512 -40486 -79.07 -329.15 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -79.07 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_2L8K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L8K-query.scw PDB file : Tito_Scwrl_2L8K.pdb: