Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALIRKRRLTEQQQRRIEKQHKTRQEEVDTSQDLDGLVVQHYGRQLEVQALSVPAHHPEKPQVAEGEPEPFWKPIELNSVWRCHTRTN-L---ELLVTGDRVKWQADPNTGLGIITAIHPRTSLLTRPDRYHKVKPVAANISLIVIVFA-PLPEPAPTLIDRYLVACADANIPALLVLNKSDLLTEN--NPILDMLKEYENLGYEVMICHS-KGD-ISALSQRLDGETVAFVGQSGVGKSSLINVLIPDAEQKTNIISENSALGQHTTTSTRLINFGRNGALIDSPGIREFGLWHLDLDKIRMGFPEIEAHLGSCQFRNCTHTHEKNCGLKQAVEAGEILPRRLDSFLRLIDEIQEAQQKN 1U0L Chain:B ((9-299)) -------------------------------LRRRGIVVSFHSNMVTVEDEET------------------------GERILCKLRGKFRLQNLKIYVGDRVEYTPDE-TGSGVIENVLHRKNLLTKPH--------VANVDQVILVVTVKMPETSTYIIDKFLVLAEKNELETVMVINKMDLYDEDDLRKVRELEEIYS-GLYPIVKTSAKTGMGIEELKEYLKGKISTMAGLSGVGKSSLLNAINPGLKLR-------------TTTTAQLLKFDFGGYVVDTPGFANLEINDIEPEELKHYFKEFGD--KQCFFSDCNHVDEPECGVKEAVENGEIAESRYENYVKMFYELLGRR---

General information: TITO was launched using: RESULT: Template: 1U0L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1372 24708 18.01 91.85 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 18.01 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_1U0L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U0L-query.scw PDB file : Tito_Scwrl_1U0L.pdb: