Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTDIQLNLIGRTQELFVEDIQHWNNKLIEIVSSSKFLVLGGAGSIGQAVVKEIFKRNPKKLHVVDISENNLTEVVRDIRSSFGYIDGDFQTFALDIGSAEYDAYIENDGDFDYVLNLSALKHVRSEKDPFTLM---RMIHVNILNTEKTIQQSIAKGVKKYFCVSTDKAANPVNMMGGSKRIMEMFLMRQSQYIDISTARFANVAFSDGSLLHGFNQRIQKNQPIAAP-NDIRRYFVTPKESGELCLMSCLLGENRDIFFPKLSEHLHLIT---FADIAVKYLNNLGYEPYLCESEDEARSLIQTLPKQGKWPCLFAG-STTTGEKDFEEFFTDNETLDMQRFNNLGVIKNELNIEEDKLQVFEEKINSMLLSKHWNKEEIVDLFNYMMPNFGHKETGLYLDGKM 3PVZ Chain:A ((10-386)) --------LIGRDTELFHQDINANEKELQSVVSQSRFLVLGGAGSIGQAVTKEIFKRNPQKLHVVDISENNMVELVRDIRSSFGYINGDFQTFALDIGSIEYDAFIKADGQYDYVLNLSALKHVRS-EK--DPFTLMRMIDVNVFNTDKTIQQSIDAGAKKYFCVSTDKAANPVNMMGASKRIMEMFLMRKSEEIAISTARFANVAFSDGSLLHGFNQRIQKNQPI-VAPNDIKRYFVTPQESGELCLMSCIFGENRDIFFPKLSEAL---HLISFADIAVKYLKQLGYEPHLCESEDEARELAKTLPAQGKWPCLFTSS----------EFFTDKETLDMARFDNLGIIKNDSLYQQELLELFEQKIGQMKTDRQWTKEEIVQLFFIMIPDF-------------

General information: TITO was launched using: RESULT: Template: 3PVZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1845 -28943 -15.69 -80.40 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -15.69 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_3PVZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PVZ-query.scw PDB file : Tito_Scwrl_3PVZ.pdb: