Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPIVIIGSGMAGYTLAREFRKLNPEQELVMICADDAVNYAKPTLSNAFAGKKAP-EQIPLGDAAKMSAQLNMRIEPFTWVKEILAERHELVLEKD--GIISQQPYSKLILAVGANPIRLAIAGDG-SDDIHVVNSLIDYRSFRENLAQRKDKRVVILGAGLIGCEFANDLLHSEYDVTVIDLAPQPLGRLLPSHIASAFQQNLEEAGVKFTLGTTVEKVSKINNGEDYAVTLANGQTLVADIVLSAIGLQPNISLAQSANIQTS--RGVITNSLLETNQADIYAIGDCAEVNGT------LLPYVMPIMQQARALAKTLSGQQ-TNVHYPAMPVAVKTPAAPLTVLPAPVDVDVN----W---ETE---------EFDDGM-LAKAIDNEGTLRGFVLLGATAGKQRLTLTKLVPDLIPAQA 3ICS Chain:B ((36-446)) SRKIVVVGGVAGGASVAARLRRLSEEDEIIMVERGEYISFANCGLPYYIGGVITERQKLLVQTVERMSKRFNLDIRVLSEVVKINKEEKTITIKNVTTNETYNEAYDVLILSPGAKPIVPSIPGIEEAKALFTLRNVPDTDRIKAYIDEKKPRHATVIGGGFIGVEMVENLRERGIEVTLVEMANQVMPPID-YEMAAYVHEHMKNHDVELVFEDGVDALEEN----GAVVRLKSGSVIQTDMLILAIGVQPESSLAKGAGLALGVRGTIKVNEKFQTSDPHIYAIGDAIEVKDFVTETETMIPLAWPANRQGRMLADIIHGHTDSLYKGTLGTSVAKVFDLTVATTGLNEKILKRLNIPYEVVHVQANSHAGYYPNATPVLIKLIFNKDSGKIYGAQTLGRDGVDKRMDVIATAI-------

General information: TITO was launched using: RESULT: Template: 3ICS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2138 -31466 -14.72 -82.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -14.72 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_3ICS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ICS-query.scw PDB file : Tito_Scwrl_3ICS.pdb: