Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRTPISFEFFPPKTDASAEKLRIVHQELQHLNPEF--FSITYGAGGSTRERTLAAIEDFNG-KGTPVAPHLSCIGDDKTRIAELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQAGANAGITQFFFNPDSYFYFIERLEKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHEVVVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN 3FST Chain:C ((22-292)) ---QINVSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANSGERDRT--HSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGD----------EMYASDLVTLLKE-VA-DFDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKLADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGV-

General information: TITO was launched using: RESULT: Template: 3FST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1333 -32659 -24.50 -125.61 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -24.50 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.669

(partial model without unconserved sides chains): PDB file : Tito_3FST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FST-query.scw PDB file : Tito_Scwrl_3FST.pdb: