Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MVFYIPKLDVMTKSTENIEKKIEAQLEKIKQLKAQKQAIEARERTKQKEQERKDDTRRKILLGS-----YLIKKMNANEANREKILAEFNEYLTDERDRKLFNL 5EQJ Chain:B ((4-376)) NCFSGYKDLIKEGDLTLIWVSRDNIKPVRMHSEEVFNTRYGSFPHKDIIGKPYGSQIAIRTFAFVHVLQPTPELWTLSLPTQIVYTPDSSYIMQRLNCSPHSRVIEAGTGSGSFSHAFARSVGHLFSFEFHHIRYEQALEEFKEHGLIDDNVTITHRDVCQGGFLIKKGDTTSYEFGNNETAASLNANVVFLDLPAPWDAIPHLDSVISVDEKVGLCCFSPCIEQVDKTLDVLEKYGWTDVEMVEIQGRQYESRRQMVRSLNDALERLRDIKRHIKEGDSNYKWKEVTKMEAEIKSHTSYLTFAFKVVNRSRDDEKVNE

General information: TITO was launched using: RESULT: Template: 5EQJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 366 53691 146.70 542.33 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : 146.70 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_5EQJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EQJ-query.scw PDB file : Tito_Scwrl_5EQJ.pdb: