Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAVMISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMAGLGAHSVIVTNACGGVNESFVPGDLMLITDHIN---FTGENPLIGPNEDAMGPRFPDMSQAYTPDYQATAKAAAEKLG--LDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEVIVAAHSGLKVLGISCITNLAAGMQANL---SHEEVVETTQRVKQSFKALVKETLTLL 1RCT Chain:E ((7-274)) -----EDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFV-------

General information: TITO was launched using: RESULT: Template: 1RCT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1388 -137818 -99.29 -530.07 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -99.29 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_1RCT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RCT-query.scw PDB file : Tito_Scwrl_1RCT.pdb: