Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPELAKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIRGDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK 3BBF Chain:B ((3-135)) LERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKP------------

General information: TITO was launched using: RESULT: Template: 3BBF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 609 -81784 -134.29 -614.91 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -134.29 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.589

(partial model without unconserved sides chains): PDB file : Tito_3BBF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BBF-query.scw PDB file : Tito_Scwrl_3BBF.pdb: