Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSNSVVNAELILTNDSNLA----------GTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFE---DALTAYIEENYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYTREVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSL-DSLGEK-KDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVFIGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDK--GQRIYFSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAW--ARRSYYQNYPDSKCNEKNLQHFYNGATMQQEFYLVDSVLTAD---------ILEAVFDKQAKDLLNGR--SDAETKKIALIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK 4K70 Chain:A ((2-483)) PGSMEALVRALEEADHAVATVVQSRILEFFMAAGRETPAGVRGLWARALRLACRAYVETGT-CEAAVLAENLAGLALWRLRHDWDEGTAPLLELLGVVNG-DDTTAALTEAG-LRTSAEFGPDAMFRLVSEWCAAFDEALAGARSADDVLAAPRVVPPEQTARALV---QPRFATLY-DMDFVQDGLRYVAQHTNWALPLALAVRQMQN--------EGLKPLTRALF-ALTIADEFFHDRQNPTLREQFAEAARAVDEAALVPVGEVNATPRTAVEVRVSAALAHGD-----------AYVRE------------LRPGTVARRL-----RTDQGVLALLDPGAQAVHVAAAADLDHTQVDATGVWEAVQASASPLQVVEALVTAG-----FTRRHCDLLERAVL---DRAPRLTDAQRAVGCTAVVGGVVHRLLDDYGPGLDYVRAYTDVADTLEPLYGDVTAALGLPEKGVEHVVRHCMAPRPPTEHVGAARAALLREVAAAERRAGLAHSAAREALNTWLAFRAQSRWGL---

General information: TITO was launched using: RESULT: Template: 4K70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2283 69028 30.24 158.32 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 30.24 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.137

(partial model without unconserved sides chains): PDB file : Tito_4K70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K70-query.scw PDB file : Tito_Scwrl_4K70.pdb: